Anticorps Polyclonal de lapin anti-PI3 Kinase p110 Alpha

PI3 Kinase p110 Alpha Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 27921-1-AP

Synonymes

p110 alpha, PI3 kinase subunit alpha, PI3K, PI3K alpha, PI3K p110(alpha), PIK3CA, PtdIns 3 kinase subunit alpha

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Galerie de données de validation

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  WB analysis using 27921-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27921-1-AP (PI3K p110(alpha) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27921-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27921-1-AP (PI3 Kinase p110 Alpha antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules NIH/3T3, tissu cérébral de rat, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below

Informations sur le produit

27921-1-AP cible PI3 Kinase p110 Alpha dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PI3 Kinase p110 Alpha Protéine recombinante Ag27539
• Nom complet: phosphoinositide-3-kinase, catalytic, alpha polypeptide
• Masse moléculaire calculée: 1068 aa, 124 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC113601
• Symbole du gène: PIK3CA
• Identification du gène (NCBI): 5290
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).

Publications

Species	Application	Title
rat	WB	J Ethnopharmacol; Xiao-Luo-Wan treats propylthiouracil-induced goiter with hypothyroidism in rats through the PI3K-AKT/RAS pathways based on UPLC/MS and network pharmacology.; Authors - Shi Dong
human	WB	Am J Cancer Res; miR-19a mediates the mechanism by which SPHK2 regulates hypopharyngeal squamous cell carcinoma progression through the PI3K/AKT axis; Authors - Ji Song
human	WB	Elife; A cleaved METTL3 potentiates the METTL3-WTAP interaction and breast cancer progression; Authors - Chaojun Yan
	WB	Eur J Pharmacol; Doxycycline hydrochloride inhibits the progress of malignant rhabdoid tumor of kidney by targeting MMP17 and MMP1 through PI3K-Akt signaling pathway; Authors - Tao Mi
mouse	WB	J Ethnopharmacol; Dendrobium huoshanense in the treatment of ulcerative colitis: Network pharmacology and experimental validation; Authors - Liang Yao
mouse,rat	WB	Curr Med Chem; Argininyl-Fructosyl-Galactose: A Novel Amino Acid Derivative improves Cisplatin-induced Intestinal Toxicity via Reactive Oxygen Species- mediated Apoptosis Pathway; Authors - Wei Liu