Anticorps Polyclonal de lapin anti-PI3 Kinase p85 Beta

PI3 Kinase p85 Beta Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 25868-1-AP

Synonymes

p85, p85 BETA, P85B, PI3 Kinase p85 Beta, PI3 kinase subunit p85 beta, PI3K p85(beta), PI3K regulatory subunit beta, PIK3R2

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Galerie de données de validation

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  WB analysis using 25868-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25868-1-AP (PI3 Kinase p85 Beta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 25868-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 25868-1-AP (PI3 Kinase p85 Beta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 25868-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25868-1-AP (PI3 Kinase p85 Beta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 25868-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25868-1-AP (PI3 Kinase p85 Beta antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 25868-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 25868-1-AP (PI3 Kinase p85 Beta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 25868-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 25868-1-AP (PI3 Kinase p85 Beta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 25868-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 25868-1-AP (PI3 Kinase p85 Beta antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 25868-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 25868-1-AP (PI3 Kinase p85 Beta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 25868-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 25868-1-AP (PI3 Kinase p85 Beta antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules U-937
• Résultats positifs en IHC: tissu placentaire humain, tissu d'intestin grêle de rat, tissu d'intestin grêle de souris, tissu rénal de rat, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

25868-1-AP cible PI3 Kinase p85 Beta dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PI3 Kinase p85 Beta Protéine recombinante Ag14369
• Nom complet: phosphoinositide-3-kinase, regulatory subunit 2 (beta)
• Masse moléculaire calculée: 728 aa, 82 kDa
• Poids moléculaire observé: 82 kDa
• Numéro d’acquisition GenBank: BC014170
• Symbole du gène: PI3 Kinase p85 Beta
• Identification du gène (NCBI): 5296
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PI3 Kinase p85 is also named as PIK3R2 and belongs to the PI3K p85 subunit family. PI3 Kinase p85 is a regulatory subunit of phosphoinositide-3-kinase (PI3K) which is a kinase that phosphorylates phosphatidylinositol 4,5-bisphosphate to generate PIP3. PI3 Kinase p85 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain, and then acts as an adapter to mediate the association of the p110 catalytic unit to the plasma membrane. It promotes nuclear translocation of XBP1 isoform 2 in a ER stress or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PMID:23604317).

Publications

Species	Application	Title
mouse	WB	Front Pharmacol; Aconiti Lateralis Radix Praeparata ameliorates heart failure via PI3K/AKT/Bnip3 pathway; Authors - Wenxiu Liu
rat	WB	Nanomaterials (Basel); 2D MXene Nanosheets with ROS Scavenging Ability Effectively Delay Osteoarthritis Progression; Authors - Hongqi Zhao
human	WB	Int J Mol Sci; miR-212-5p Regulates PM2.5-Induced Apoptosis by Targeting LAMC2 and LAMA3; Authors - Yunna Jia

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