Anticorps Polyclonal de lapin anti-PI4KA

• Phare
• Validé par KD/KO

PI4KA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(12)

Avis (1)

N° de cat : 12411-1-AP

Synonymes

EC:2.7.1.67, Phosphatidylinositol 4-kinase alpha, Phosphatidylinositol 4-Kinase III alpha, PI4K-alpha, PI4-kinase alpha

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Galerie de données de validation

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  WB analysis of MCF-7 using 12411-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12411-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH-3T3 cells (RNAi) using 12411-1-AP

  WB result of anti- PI4KA (12411-1-AP) with NIH-3T3 cells (RNAi).

• 

  WB analysis of human brain using 12411-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12411-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12411-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12411-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12411-1-AP (PI4KA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12411-1-AP

  IP result of anti-PI4KA (IP:12411-1-AP, 4ug; Detection:12411-1-AP 1:3000) with mouse brain tissue lysate 1080 ug.

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  IHC staining of human placenta using 12411-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12411-1-AP (PI4KA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 12411-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12411-1-AP (PI4KA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 12411-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12411-1-AP (PI4KA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 12411-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PI4KA antibody (12411-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7, cellules NIH-3T3 (ARNi), tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu placentaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

12411-1-AP cible PI4KA dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PI4KA Protéine recombinante Ag3062
• Nom complet: phosphatidylinositol 4-kinase, catalytic, alpha
• Masse moléculaire calculée: 2044 aa, 231 kDa
• Poids moléculaire observé: 231 kDa
• Numéro d’acquisition GenBank: BC018120
• Symbole du gène: PI4KA
• Identification du gène (NCBI): 5297
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PI4KA, also named as PIK4, PIK4CA, belongs to the PI3/PI4-kinase family and Type III PI4K subfamily. It acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate. PI4KA plays a role in the formation of membrane complexes where HCV replication takes place. (PMID:19605471).

Publications

Species	Application	Title
human	WB	Nature; A phosphoinositide signalling pathway mediates rapid lysosomal repair; Authors - Jay Xiaojun Tan ; KD Validated
human	WB	Science; Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.; Authors - Shun Nagashima ; KD Validated
human	WB,IF	Brain; Biallelic PI4KA variants cause a novel neurodevelopmental syndrome with hypomyelinating leukodystrophy.; Authors - Edgard Verdura
human	IF	EMBO J; Defining the proximal interaction networks of Arf GTPases reveals a mechanism for the regulation of PLD1 and PI4KB.; Authors - Zhengming Wu
mouse,human	WB,IHC,IF	Cell Rep; A Forward Genetic Screen Targeting the Endothelium Reveals a Regulatory Role for the Lipid Kinase Pi4ka in Myelo- and Erythropoiesis.; Authors - Safiyyah Ziyad ; KD Validated
human	WB	Biomaterials; Nrf-2-driven long noncoding RNA ODRUL contributes to modulating silver nanoparticle-induced effects on erythroid cells.; Authors - Ming Gao ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Patricia (Verified Customer) (06-16-2024)

Works well with 30ug of cell lysate

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: immune cells

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