Anticorps Polyclonal de lapin anti-PIAS1

• Phare
• Validé par KD/KO

PIAS1 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, IP, coIP, ELISA

Conjugaison

Non conjugué

Publications(12)

Avis (1)

N° de cat : 23395-1-AP

Synonymes

DDXBP1, DEAD/H box-binding protein 1, E3 SUMO-protein ligase PIAS1, E3 SUMO-protein transferase PIAS1, EC:2.3.2.-

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Galerie de données de validation

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  WB analysis using 23395-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23395-1-AP (PIAS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 23395-1-AP

  IP result of anti-PIAS1 (IP:23395-1-AP, 4ug; Detection:23395-1-AP 1:500) with HepG2 cells lysate 2520 ug.

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  IHC staining of mouse testis using 23395-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23395-1-AP (PIAS1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 23395-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23395-1-AP (PIAS1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules Jurkat, cellules L02, cellules RAW 264.7
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

23395-1-AP cible PIAS1 dans les applications de WB, IHC, IF, IP, coIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PIAS1 Protéine recombinante Ag18132
• Nom complet: protein inhibitor of activated STAT, 1
• Masse moléculaire calculée: 651 aa, 72 kDa
• Poids moléculaire observé: 72 kDa
• Numéro d’acquisition GenBank: BC121797
• Symbole du gène: PIAS1
• Identification du gène (NCBI): 8554
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; CryoET reveals organelle phenotypes in huntington disease patient iPSC-derived and mouse primary neurons; Authors - Gong-Her Wu
human	WB	Nat Commun; Crosstalk between SUMOylation and ubiquitylation controls DNA end resection by maintaining MRE11 homeostasis on chromatin; Authors - Tao Zhang
rat,mouse	WB,coIP	J Hazard Mater; Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles.; Authors - Junrong Wu
human	WB,IF	PLoS Pathog; PIAS1-mediated SUMOylation of influenza A virus PB2 restricts viral replication and virulence.; Authors - Guangwen Wang ; KD Validated
human	WB	Mol Med Rep; Role of SUMOylation of STAT1 in tubular epithelial‑mesenchymal transition induced by high glucose; Authors - Cunyang Gu
human	WB,IHC	DNA Cell Biol; miR-182-5p and miR-96-5p Target PIAS1 and Mediate the Negative Feedback Regulatory Loop between PIAS1 and STAT3 in Endometrial Cancer.; Authors - Yuzhen Xiao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Praveen (Verified Customer) (02-25-2019)

WORK WELL

• Applications: Immunoprecipitation,
• Cell Tissue Type: Ac16

PIAS1 Antibody Immunoprecipitation, validation ( dilution) in Ac16 (Cat no:23395-1-AP)

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