Anticorps Recombinant de lapin anti-PIAS1

PIAS1 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C22

N° de cat : 82873-2-PBS

Synonymes

EC:2.3.2.-, E3 SUMO-protein transferase PIAS1, E3 SUMO-protein ligase PIAS1, DEAD/H box-binding protein 1, DDXBP1

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Galerie de données de validation

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  WB analysis using 82873-2-RR (same clone as 82873-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82873-2-RR (PIAS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82873-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 82873-2-RR (same clone as 82873-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82873-2-RR (PIAS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82873-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 82873-2-RR (same clone as 82873-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82873-2-RR (PIAS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82873-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82873-2-RR (same clone as 82873-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PIAS1 antibody (82873-2-RR, Clone: 1C22 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82873-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82873-2-RR (same clone as 82873-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug PIAS1 Recombinant antibody (82873-2-RR, Clone:1C22) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82873-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82873-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82873-2-RR against Human PIAS1 were performed. The affinity constant is 5.01 nM.

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Informations sur le produit

82873-2-PBS cible PIAS1 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PIAS1 Protéine recombinante Ag18132
• Nom complet: protein inhibitor of activated STAT, 1
• Masse moléculaire calculée: 651 aa, 72 kDa
• Poids moléculaire observé: 72 kDa
• Numéro d’acquisition GenBank: BC121797
• Symbole du gène: PIAS1
• Identification du gène (NCBI): 8554
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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