Anticorps Recombinant de lapin anti-PIAS2

PIAS2 Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241474G8

N° de cat : 84189-4-PBS

Synonymes

241474G8, Androgen receptor-interacting protein 3, ARIP3, DAB2-interacting protein, DIP

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Galerie de données de validation

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  WB analysis of mouse testis using 84189-4-RR (same clone as 84189-4-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 84189-4-RR (PIAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84189-4-PBS in a different storage buffer formulation.

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  WB analysis using 84189-4-RR (same clone as 84189-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84189-4-RR (PIAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84189-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01062-1

  Cytometric bead array standard curve of MP01062-1, PIAS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84189-4-PBS. Detection antibody: 84189-1-PBS. Standard: Ag9032. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP01062-2

  Cytometric bead array standard curve of MP01062-2, PIAS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84189-4-PBS. Detection antibody: 84189-2-PBS. Standard: Ag9032. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84189-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84189-4-RR against Human PIAS2 were performed. The affinity constant is 0.28 nM.

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Informations sur le produit

84189-4-PBS cible PIAS2 dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PIAS2 Protéine recombinante Ag9032
• Nom complet: protein inhibitor of activated STAT, 2
• Masse moléculaire calculée: 63 kDa, 69 kDa
• Poids moléculaire observé: 63-68 kDa
• Numéro d’acquisition GenBank: BC015190
• Symbole du gène: PIAS2
• Identification du gène (NCBI): 9063
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PIAS2 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. PIAS2 also plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. PIAS2 has 3 isoforms produced by alternative splicing with the MW of 68 kDa, 63 kDa and 45 kDa.

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