Anticorps Recombinant de lapin anti-PIAS2

PIAS2 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241474G8

N° de cat : 84189-4-RR

Synonymes

E3 SUMO-protein ligase PIAS2, DIP, DAB2-interacting protein, ARIP3, Androgen receptor-interacting protein 3

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Galerie de données de validation

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  WB analysis of mouse testis using 84189-4-RR

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 84189-4-RR (PIAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 84189-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84189-4-RR (PIAS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84189-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84189-4-RR against Human PIAS2 were performed. The affinity constant is 0.28 nM.

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HEK-293T, cellules HeLa, tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84189-4-RR cible PIAS2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PIAS2 Protéine recombinante Ag9032
• Nom complet: protein inhibitor of activated STAT, 2
• Masse moléculaire calculée: 63 kDa, 69 kDa
• Poids moléculaire observé: 63-68 kDa
• Numéro d’acquisition GenBank: BC015190
• Symbole du gène: PIAS2
• Identification du gène (NCBI): 9063
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PIAS2 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. PIAS2 also plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. PIAS2 has 3 isoforms produced by alternative splicing with the MW of 68 kDa, 63 kDa and 45 kDa.

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