Anticorps Polyclonal de lapin anti-PIBF1

• Phare
• Validé par KD/KO

PIBF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14413-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 14413-1-AP (same clone as 14413-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14413-1-AP (PIBF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14413-1-PBS in a different storage buffer formulation.

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  WB analysis using 14413-1-AP (same clone as 14413-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14413-1-AP (PIBF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14413-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 14413-1-AP (same clone as 14413-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14413-1-AP (PIBF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14413-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 14413-1-AP (same clone as 14413-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 14413-1-AP (PIBF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14413-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 14413-1-AP (same clone as 14413-1-PBS)

  IP result of anti-PIBF1 (IP:14413-1-AP, 4ug; Detection:14413-1-AP 1:1000) with HEK-293 cells lysate 1400 ug. This data was developed using the same antibody clone with 14413-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 14413-1-AP (same clone as 14413-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 14413-1-AP (PIBF1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14413-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 14413-1-AP (same clone as 14413-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using PIBF1 antibody (14413-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Gamma Tubulin antibody (66320-1-Ig, Clone: 3F9H8, red). This data was developed using the same antibody clone with 14413-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14413-1-PBS cible PIBF1 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PIBF1 Protéine recombinante Ag5755
• Nom complet: progesterone immunomodulatory binding factor 1
• Masse moléculaire calculée: 90 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC051911
• Symbole du gène: PIBF1
• Identification du gène (NCBI): 10464
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PIBF1 is induced by the steroid hormone progesterone and plays a role in the maintenance of pregnancy. PIBF1 regulates multiple facets of the immune system to promote normal pregnancy including cytokine synthesis, natural killer (NK) cell activity, and arachidonic acid metabolism. Low serum levels of this protein have been associated with spontaneous pre-term labor in humans. PIBF1 may promote the proliferation, migration and invasion of glioma.

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