Anticorps Polyclonal de lapin anti-PICALM

PICALM Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28554-1-PBS

Synonymes

CALM, CLTH, LAP, Phosphatidylinositol-binding clathrin assembly protein

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Galerie de données de validation

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  WB analysis using 28554-1-AP (same clone as 28554-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28554-1-AP (PICALM antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28554-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 28554-1-AP (same clone as 28554-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28554-1-AP (PICALM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28554-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 28554-1-AP (same clone as 28554-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PICALM antibody (28554-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28554-1-PBS in a different storage buffer formulation.

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Informations sur le produit

28554-1-PBS cible PICALM dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PICALM Protéine recombinante Ag29505
• Nom complet: phosphatidylinositol binding clathrin assembly protein
• Masse moléculaire calculée: 71 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC064357
• Symbole du gène: PICALM
• Identification du gène (NCBI): 8301
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PICALM (Phosphatidyl Inositol Clathrin Assembly Lymphoid Myeloid protein) is a ubiquitously expressed protein that plays a role in clathrin-mediated endocytosis. PICALM also affects the internalization and trafficking of SNAREs and modulates macroautophagy. PICALM is a highly validated genetic risk factor for Alzheimer's disease (AD). The long isoform of human PICALM has 22 exons (encoding PICALML), while the short isoform lacks exon 14 (encoding PICALMS). (PMID: 33397371, PMID: 26075887, PMID: 26005850)

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