Anticorps Recombinant de lapin anti-PICK1

• Phare
• Validé par KD/KO

PICK1 Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230185B8

N° de cat : 82919-1-RR

Synonymes

230185B8, PICK, PRKCA binding protein, PRKCA-binding protein, PRKCABP

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Galerie de données de validation

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  WB analysis using 82919-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82919-1-RR (PICK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 82919-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82919-1-RR (PICK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 82919-1-RR

  WB result of PICK1 antibody (82919-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PICK1 transfected HEK-293 cells.

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  IHC staining of human stomach cancer using 82919-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82919-1-RR (PICK1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 82919-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82919-1-RR (PICK1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 82919-1-RR

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 82919-1-RR (PICK1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HEK-293T using 82919-1-RR

  1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug Anti-Human PICK1 (82919-1-RR, Clone:230185B8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.25 ug rabbit IgG isotype control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82919-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82919-1-RR against Human PICK1 were performed. The affinity constant is 0.792 nM.

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HEK-293, cellules HeLa, cellules MCF-7, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HEK-293T,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82919-1-RR cible PICK1 dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PICK1 Protéine recombinante Ag1443
• Nom complet: protein interacting with PRKCA 1
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC017561
• Symbole du gène: PICK1
• Identification du gène (NCBI): 9463
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein interacting with C kinase 1 (PICK1) was first cloned as a PKC-binding partner through yeast two hybrid system. PICK1 acts as a critical regulator of membrane receptors' subcellular trafficking to modulate neural processes such as learning and memory, and is widely expressed in brain, testis, heart, lung, liver, kidney and muscle. It probably binds to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence, for instance, PICK1 is a critical mediator of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) trafficking in neural synapses. PICK1 expression on D-serine release and glutamate transport in astrocytes suggests a potential implication of PICK1 in the progression of amyotrophic lateral sclerosis (ALS). PICK1 may also participate in breast cancer development through inhibition of TGF-β signaling.

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