Anticorps Polyclonal de lapin anti-PIG3

PIG3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14828-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 14828-1-AP (same clone as 14828-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14828-1-AP (PIG3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14828-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 14828-1-AP (same clone as 14828-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14828-1-AP (PIG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14828-1-PBS in a different storage buffer formulation.

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  WB analysis of SMMC-7721 using 14828-1-AP (same clone as 14828-1-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 14828-1-AP (PIG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14828-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 14828-1-AP (same clone as 14828-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14828-1-AP (PIG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14828-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 14828-1-AP (same clone as 14828-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14828-1-AP (PIG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14828-1-PBS in a different storage buffer formulation.

• 

  IP experiment of MCF-7 using 14828-1-AP (same clone as 14828-1-PBS)

  IP result of anti-PIG3 (IP:14828-1-AP, 4ug; Detection:14828-1-AP 1:500) with MCF-7 cells lysate 3200ug. This data was developed using the same antibody clone with 14828-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 14828-1-AP (same clone as 14828-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14828-1-AP (PIG3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14828-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14828-1-AP (same clone as 14828-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14828-1-AP (PIG3 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14828-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14828-1-AP (same clone as 14828-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PIG3 antibody (14828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 14828-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14828-1-PBS cible PIG3 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PIG3 Protéine recombinante Ag6618
• Nom complet: tumor protein p53 inducible protein 3
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 34-36 kDa
• Numéro d’acquisition GenBank: BC000474
• Symbole du gène: TP53I3
• Identification du gène (NCBI): 9540
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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