Anticorps Polyclonal de lapin anti-PIGK

PIGK Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15151-1-PBS

Synonymes

Phosphatidylinositol-glycan biosynthesis class K protein, hGPI8, GPI-anchor transamidase, GPI8, GPI transamidase

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Galerie de données de validation

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  WB analysis of HT-1080 using 15151-1-AP (same clone as 15151-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 15151-1-AP (PIGK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15151-1-PBS in a different storage buffer formulation.

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  WB analysis of SMMC-7721 using 15151-1-AP (same clone as 15151-1-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 15151-1-AP (PIGK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15151-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 15151-1-AP (same clone as 15151-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15151-1-AP (PIGK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15151-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 15151-1-AP (same clone as 15151-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15151-1-AP (PIGK antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15151-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 15151-1-AP (same clone as 15151-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15151-1-AP (PIGK antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15151-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15151-1-PBS cible PIGK dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PIGK Protéine recombinante Ag3497
• Nom complet: phosphatidylinositol glycan anchor biosynthesis, class K
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC026186
• Symbole du gène: PIGK
• Identification du gène (NCBI): 10026
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Phosphatidylinositol glycan, class K (PIGK), is a crucial member of the glycosyl-phosphatidylinositol transamidase (GPIT) protein complex that attaches a diverse group of macromolecules to the plasma membrane of eukaryotes. The human PIGK gene is involved in the key step of transferring GPI-anchor to the respective protein molecules in the plasma membrane (PMID: 22824918). Endogenous PIGK has 2 isoforms, 45 kDa and 36 kDa (PMID: 34193731).

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