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Anticorps Recombinant de lapin anti-PIGK

PIGK Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241989D4

N° de cat : 84568-3-PBS

Synonymes

hGPI8, GPI-anchor transamidase, GPI8, GPI transamidase, EC:3.-.-.-

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using PIGK Recombinant antibody (84568-3-RR)

    WB analysis using 84568-3-RR (same clone as 84568-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84568-3-RR (PIGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84568-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84568-3-RR

    Affinity and Kinetic Characterization of 84568-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84568-3-RR against Human PIGK were performed. The affinity constant is 0.501 nM.

Informations sur le produit

84568-3-PBS cible PIGK dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène PIGK Protéine recombinante Ag3497
Nom complet phosphatidylinositol glycan anchor biosynthesis, class K
Masse moléculaire calculée 45 kDa
Poids moléculaire observé40-45 kDa
Numéro d’acquisition GenBankBC026186
Symbole du gène PIGK
Identification du gène (NCBI) 10026
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Protein A purfication
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Phosphatidylinositol glycan, class K (PIGK), is a crucial member of the glycosyl-phosphatidylinositol transamidase (GPIT) protein complex that attaches a diverse group of macromolecules to the plasma membrane of eukaryotes. The human PIGK gene is involved in the key step of transferring GPI-anchor to the respective protein molecules in the plasma membrane (PMID: 22824918). Endogenous PIGK has 2 isoforms, 45 kDa and 36 kDa (PMID: 34193731).

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