Anticorps Polyclonal de lapin anti-PIGS

PIGS Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18334-1-PBS

Synonymes

UNQ1873/PRO4316, Phosphatidylinositol-glycan biosynthesis class S protein, GPI transamidase component PIG-S

---

Galerie de données de validation

• 

  WB analysis of HeLa using 18334-1-AP (same clone as 18334-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 18334-1-AP (PIGS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18334-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 18334-1-AP (same clone as 18334-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18334-1-AP (PIGS Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 18334-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 18334-1-AP (same clone as 18334-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18334-1-AP (PIGS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18334-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 18334-1-AP (same clone as 18334-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 18334-1-AP (PIGS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18334-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 18334-1-AP (same clone as 18334-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18334-1-AP (PIGS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18334-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 18334-1-AP (same clone as 18334-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PIGS antibody (18334-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18334-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

18334-1-PBS cible PIGS dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PIGS Protéine recombinante Ag12950
• Nom complet: phosphatidylinositol glycan anchor biosynthesis, class S
• Masse moléculaire calculée: 62 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC001319
• Symbole du gène: PIGS
• Identification du gène (NCBI): 94005
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PIGS, also named as GPI transamidase component PIG-S or PRO4316, is a 555 amino acid protein, which belongs to the PIGS family. PIGS localizes in the endoplasmic reticulum membrane. PIGS is a component of the GPI transamidase complex. It is essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates.

{{ptg:RelatedPrimaryAntibodies}}