Anticorps Polyclonal de lapin anti-PIGT

• Phare
• Validé par KD/KO

PIGT Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 16906-1-AP

Synonymes

CGI-06, GPI transamidase component PIG-T, NDAP, Phosphatidylinositol-glycan biosynthesis class T protein, PSEC0163

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Galerie de données de validation

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  WB analysis of human brain using 16906-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16906-1-AP (PIGT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 16906-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16906-1-AP (PIGT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 16906-1-AP

  IP result of anti-PIGT (IP:16906-1-AP, 4ug; Detection:16906-1-AP 1:300) with mouse liver tissue lysate 6000 ug.

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  IHC staining of human brain using 16906-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16906-1-AP (PIGT antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 16906-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16906-1-AP (PIGT antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse brain using 16906-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16906-1-AP (PIGT antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16906-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PIGT antibody (16906-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules A549
• Résultats positifs en IP: tissu hépatique de souris,
• Résultats positifs en IHC: tissu cérébral humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

16906-1-AP cible PIGT dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PIGT Protéine recombinante Ag10521
• Nom complet: phosphatidylinositol glycan anchor biosynthesis, class T
• Masse moléculaire calculée: 578 aa, 66 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC015022
• Symbole du gène: PIGT
• Identification du gène (NCBI): 51604
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PIGT is a subunit of the glycosylphosphatidylinositol transamidase complex that catalyzes the attachment of proteins to GPI-anchors. GPI-anchors are glycolipids found on membrane of diverse cells that help proteins anchoring to the cell surface. Mutations of PIGT have been linked to developmental disorders including multiple congenital anomalies-hypotonia-seizures syndrome-3. Multiple isoforms of PIGT exist due to the alternative splicing, with the predicted MWs ranging from 42 kDa to 66 kDa.

Publications

Species	Application	Title
human	WB	J Transl Med; PIGT promotes cell growth, glycolysis, and metastasis in bladder cancer by modulating GLUT1 glycosylation and membrane trafficking; Authors - Mingyue Tan ; KD Validated
mouse	WB,IF	CNS Neurosci Ther; Integrated transcriptomic and proteomic profiling reveals the key molecular signatures of brain endothelial reperfusion injury; Authors - Yabin Ji
mouse,human	WB	Nat Commun; Proteomic screens of SEL1L-HRD1 ER-associated degradation substrates reveal its role in glycosylphosphatidylinositol-anchored protein biogenesis; Authors - Xiaoqiong Wei
mouse	WB	BMC Med Genomics; Proteomic analysis illustrates the potential involvement of dysregulated ribosome-related pathways and disrupted metabolism during retinoic acid-induced cleft palate development; Authors - Wancong Zhang

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