Anticorps Polyclonal de lapin anti-PIH1D1

PIH1D1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 19427-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 19427-1-AP (same clone as 19427-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 19427-1-AP (same clone as 19427-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse eye using 19427-1-AP (same clone as 19427-1-PBS)

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

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  WB analysis of human uterus using 19427-1-AP (same clone as 19427-1-PBS)

  human uterus tissue were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 19427-1-AP (same clone as 19427-1-PBS)

  IP result of anti-PIH1D1 (IP:19427-1-AP, 4ug; Detection:19427-1-AP 1:1000) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 19427-1-AP (same clone as 19427-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 19427-1-AP (same clone as 19427-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary using 19427-1-AP (same clone as 19427-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary using 19427-1-AP (same clone as 19427-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 19427-1-AP (same clone as 19427-1-PBS)

  Immunofluorescent analysis of Hela cells, using PIH1D1 antibody 19427-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

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Informations sur le produit

19427-1-PBS cible PIH1D1 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PIH1D1 Protéine recombinante Ag13759
• Nom complet: PIH1 domain containing 1
• Masse moléculaire calculée: 290 aa, 32 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC001108
• Symbole du gène: PIH1D1
• Identification du gène (NCBI): 55011
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PIH1D1 (PIH1 domain-containing protein 1; also known as PIH1 or NOP17), is a ortholog of the yeast nucleolar protein NOP17. PIH1D1 is a conserved subunit of the R2TP complex participating in the assembly of snoRNP and RNA polymerase II complexes. PIH1D1 may also function as a novel modulator of apoptosis pathway.

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