Anticorps Polyclonal de lapin anti-VPS34

• Phare
• Validé par KD/KO

VPS34 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 13723-1-AP

Synonymes

PIK3C3, EC:2.7.1.137, hVps34, PI 3 Kinase Class 3, PI 3-kinase p100

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Galerie de données de validation

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  WB analysis of HeLa using 13723-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13723-1-AP (VPS34 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13723-1-AP

  WB result of VPS34 antibody (13723-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS34 transfected Jurkat cells.

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  WB analysis of mouse testis using 13723-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13723-1-AP (VPS34 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 13723-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13723-1-AP (VPS34 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 13723-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13723-1-AP (VPS34 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 13723-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13723-1-AP (VPS34 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, tissu pulmonaire de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de muscle squelettique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below

Informations sur le produit

13723-1-AP cible VPS34 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: phosphoinositide-3-kinase, class 3
• Masse moléculaire calculée: 887 aa, 100 kDa
• Poids moléculaire observé: 102 kDa
• Numéro d’acquisition GenBank: BC053651
• Symbole du gène: VPS34
• Identification du gène (NCBI): 5289
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PIK3C3(Phosphatidylinositol 3-kinase catalytic subunit type 3) is also named as VPS34 and belongs to the PI3/PI4-kinase family.It is a catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes.It can form a dimer(PMID:20339072).

Publications

Species	Application	Title
pig	WB	J Virol; Potassium molybdate blocks APN-dependent coronavirus entry by degrading receptor via PIK3C3-mediated autophagy; Authors - Yunhang Zhang ; KD Validated
mouse	WB	Front Pharmacol; Integrated Network Pharmacology and Lipidomics to Reveal the Inhibitory Effect of Qingfei Oral Liquid on Excessive Autophagy in RSV-Induced Lung Inflammation.; Authors - Lili Lin
rat	WB	J Cardiovasc Transl Res; The Protective Mechanism of Dexmedetomidine in Regulating Atg14L-Beclin1-Vps34 Complex Against Myocardial Ischemia-Reperfusion Injury.; Authors - Yanna Li
human	WB	Nat Struct Mol Biol; Reduction of DHHC5-mediated beclin 1 S-palmitoylation underlies autophagy decline in aging; Authors - Rui Guo

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