Anticorps Polyclonal de lapin anti-PI3 Kinase p110 Alpha

PI3 Kinase p110 Alpha Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 20583-1-AP

Synonymes

p110 alpha, PI3 Kinase p110 Alpha, PI3 kinase subunit alpha, PI3K, PI3K alpha, PI3K p110(alpha), PI3-kinase p110 subunit alpha, PIK3CA, PtdIns 3 kinase subunit alpha

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Galerie de données de validation

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  WB analysis of HepG2 using 20583-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20583-1-AP (PI3K p110(alpha) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20583-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20583-1-AP (PI3K p110(alpha) antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 20583-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20583-1-AP (PI3K p110(alpha) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 20583-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20583-1-AP (PI3K p110(alpha) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20583-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20583-1-AP (PI3K p110(alpha) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20583-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20583-1-AP (PI3K p110(alpha) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 20583-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20583-1-AP (PI3K p110(alpha) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 20583-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20583-1-AP (PI3K p110(alpha) antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 20583-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20583-1-AP (PI3K p110(alpha) antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 20583-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20583-1-AP (PI3K p110(alpha) antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A431, cellules COLO 320, cellules HeLa, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:300-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 3 publications below

Informations sur le produit

20583-1-AP cible PI3 Kinase p110 Alpha dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: phosphoinositide-3-kinase, catalytic, alpha polypeptide
• Masse moléculaire calculée: 124 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: NM_006218
• Symbole du gène: PIK3CA
• Identification du gène (NCBI): 5290
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB). The antibody is specific to PIK3CA.

Publications

Species	Application	Title
human	WB,IHC	Oncogene; KIF26B, a novel oncogene, promotes proliferation and metastasis by activating the VEGF pathway in gastric cancer.; Authors - H Zhang
human	WB	Oncotarget; Epithelial but not stromal expression of collagen alpha-1(III) is a diagnostic and prognostic indicator of colorectal carcinoma.; Authors - Xiao-Qing Wang
human	IHC	J Cancer; Vasculogenic Mimicry in Prostate Cancer: The Roles of EphA2 and PI3K.; Authors - Hua Wang
mouse	WB	Mol Med Rep; Resveratrol improves palmitic acid‑induced insulin resistance via the DDIT4/mTOR pathway in C2C12 cells; Authors - Xinyan Pan
	WB	Dev Comp Immunol; Characterization, functional and signaling elucidation of pigeon (Columba livia) interferon-α: Knockdown p53 negatively modulates antiviral response.; Authors - Siwen Li
		Biomed Pharmacother; Mitochondrial DNA content reduction induces aerobic glycolysis and reversible resistance to drug-induced apoptosis in SW480 colorectal cancer cells.; Authors - Jing-Jing Mou

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