Anticorps Monoclonal anti-PI3 Kinase p110 Beta

PI3 Kinase p110 Beta Monoclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

21

CloneNo.

1B2A1

N° de cat : 67121-1-Ig

Synonymes

DKFZp779K1237, p110 BETA, PI3 kinase subunit beta, PI3K, PI3K beta, PI3K p110(beta), PI3Kbeta, PI3KCB, PIK3C1, PIK3CB, PtdIns 3 kinase subunit beta

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Galerie de données de validation

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  WB analysis using 67121-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67121-1-Ig (PIK3CB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67121-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67121-1-Ig (PIK3CB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67121-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67121-1-Ig (PIK3CB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67121-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67121-1-Ig (PIK3CB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 67121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67121-1-Ig (PIK3CB antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 67121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67121-1-Ig (PIK3CB antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67121-1-Ig (PI3 Kinase p110 Beta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67121-1-Ig (PI3 Kinase p110 Beta antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 67121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67121-1-Ig (PI3 Kinase p110 Beta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 67121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67121-1-Ig (PI3 Kinase p110 Beta antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67121-1-Ig (PI3 Kinase p110 Beta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67121-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67121-1-Ig (PI3 Kinase p110 Beta antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules MCF-7, cellules PC-12, cellules SH-SY5Y
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du col de l'utérus humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 19 publications below
• IHC: See 4 publications below

Informations sur le produit

67121-1-Ig cible PI3 Kinase p110 Beta dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PI3 Kinase p110 Beta Protéine recombinante Ag17505
• Nom complet: phosphoinositide-3-kinase, catalytic, beta polypeptide
• Masse moléculaire calculée: 1070 aa, 123 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: BC114432
• Symbole du gène: PIK3CB
• Identification du gène (NCBI): 5291
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PIK3CB(phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform) is also named as PIK3C1, PI3K-beta, p110beta. The gene encodes a 1070 amino acid protein which belongs to the PI3/PI4-kinase family. Phosphoinositide 3-kinases (PI3Ks) have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. The class I PI3 kinases are heterodimers composed of 110 kDa catalytic subunits that associate with regulatory adaptor proteins. Four class I catalytic subunits have been identified, PIK3CA (p110α), PIK3CB (p110β), PIK3CD (p110δ) and PIK3CG (p110γ)(PMID:19177002).

Publications

Species	Application	Title
human	WB	J Med Chem; Discovery of Novel Phosphoinositide-3-Kinase α Inhibitors with High Selectivity, Excellent Bioavailability, and Long-Acting Efficacy for Gastric Cancer.; Authors - Yi Hou
human	WB	J Ethnopharmacol; Antitumor mechanism of kangliu pill on gliomas in mice through PI3K-Akt signaling pathway; Authors - Fei Chen
human	WB,IHC	Front Oncol; Relationship Between PTEN and Angiogenesis of Esophageal Squamous Cell Carcinoma and the Underlying Mechanism.; Authors - Chenbo Yang
human	WB	Bioorg Chem; Design, synthesis and biological evaluation of anilide (dicarboxylic acid) shikonin esters as antitumor agents through targeting PI3K/Akt/mTOR signaling pathway.; Authors - Yingying Ma
mouse	WB	Chin Med; Integrated metabolomics, network pharmacology and biological verification to reveal the mechanisms of Nauclea officinalis treatment of LPS-induced acute lung injury; Authors - Han Xu
mouse	WB	Cells; Extracellular Vesicles from BMSCs Prevent Glucocorticoid-Induced BMECs Injury by Regulating Autophagy via the PI3K/Akt/mTOR Pathway.; Authors - Jinhui Ma