Anticorps Recombinant de lapin anti-PI3 Kinase p85 Beta

PI3 Kinase p85 Beta Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240531B5

N° de cat : 83606-5-PBS

Synonymes

PI3K p85 β, PI3K p85(beta), PI3K p85β, PIK3R2, p85β

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Galerie de données de validation

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  WB analysis using 83606-5-RR (same clone as 83606-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83606-5-RR (PIK3R2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83606-5-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 83606-5-RR (same clone as 83606-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PIK3R2 antibody (83606-5-RR, Clone: 240531B5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83606-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83606-5-RR (same clone as 83606-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PIK3R2 antibody (83606-5-RR, Clone: 240531B5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83606-5-PBS in a different storage buffer formulation.

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Informations sur le produit

83606-5-PBS cible PI3 Kinase p85 Beta dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PI3 Kinase p85 Beta Protéine recombinante Ag14367
• Nom complet: phosphoinositide-3-kinase, regulatory subunit 2 (beta)
• Masse moléculaire calculée: 728 aa, 82 kDa
• Poids moléculaire observé: 82 kDa
• Numéro d’acquisition GenBank: BC014170
• Symbole du gène: PI3 Kinase p85 Beta
• Identification du gène (NCBI): 5296
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PIK3R2 is also named as PI3 Kinase p85 and belongs to the PI3K p85 subunit family. PI3 Kinase p85 is a regulatory subunit of phosphoinositide-3-kinase (PI3K) which is a kinase that phosphorylates phosphatidylinositol 4,5-bisphosphate to generate PIP3. PI3 Kinase p85 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain, and then acts as an adapter to mediate the association of the p110 catalytic unit to the plasma membrane. It promotes nuclear translocation of XBP1 isoform 2 in a ER stress or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PMID:23604317).

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