Anticorps Polyclonal de lapin anti-VPS15

VPS15 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 17894-1-AP

Synonymes

PI3K p150, PIK3R4, PI3-kinase p150 subunit, Phosphoinositide 3-kinase regulatory subunit 4, Phosphoinositide 3-kinase adaptor protein

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Galerie de données de validation

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  WB analysis of HepG2 using 17894-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17894-1-AP (VPS15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17894-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17894-1-AP (VPS15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 17894-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VPS15 antibody (17894-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

17894-1-AP cible VPS15 dans les applications de WB, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VPS15 Protéine recombinante Ag12221
• Nom complet: phosphoinositide-3-kinase, regulatory subunit 4
• Masse moléculaire calculée: 1358 aa, 153 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC110318
• Symbole du gène: PIK3R4
• Identification du gène (NCBI): 30849
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PIK3R4(phosphoinositide 3-kinase regulatory subunit 4) is also named as PI3-kinase p150 subunit and belongs to the Ser/Thr protein kinase family. It is a regulatory subunit of the PI3K complex and may regulate membrane trafficking late in the endocytic pathway. This protein can be probably autophosphorylated(PMID:19369195).

Publications

Species	Application	Title
mouse	WB	Nat Neurosci; Mutations in Vps15 perturb neuronal migration in mice and are associated with neurodevelopmental disease in humans.; Authors - Thomas Gstrein
human	WB	Nat Commun; Structural basis for VPS34 kinase activation by Rab1 and Rab5 on membranes.; Authors - Shirley Tremel
	IP,CoIP	Nat Commun; IL-6 regulates autophagy and chemotherapy resistance by promoting BECN1 phosphorylation.; Authors - Fuqing Hu
mouse	WB	Dev Cell; Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.; Authors - Yuqing Lei
rat	WB	J Hazard Mater; Nano-graphene oxide depresses neurotransmission by blocking retrograde transport of mitochondria.; Authors - Yiyuan Kang
		Cancer Lett; Targeting AR-Beclin 1 Complex-Modulated Growth Factor Signaling Increases the Antiandrogen-Enzalutamide Sensitivity to Better Suppress the Castration-Resistant Prostate Cancer Growth.; Authors - Meng Zhang

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