Anticorps Recombinant de lapin anti-PIN1

• Phare
• Validé par KD/KO

PIN1 Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5N20

N° de cat : 81857-1-PBS

Synonymes

Pin 1, Peptidyl-prolyl cis-trans isomerase Pin1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, EC:5.2.1.8, 5N20

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Galerie de données de validation

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  WB analysis using 81857-1-RR (same clone as 81857-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81857-1-RR (PIN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81857-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 81857-1-RR (same clone as 81857-1-PBS)

  WB result of PIN1 antibody (81857-1-RR; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PIN1 transfected HeLa cells. This data was developed using the same antibody clone with 81857-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 81857-1-RR (same clone as 81857-1-PBS)

  IP result of anti-PIN1 (IP:81857-1-RR, 4ug; Detection:81857-1-RR 1:20000) with HepG2 cells lysate 1920 ug. This data was developed using the same antibody clone with 81857-1-PBS in a different storage buffer formulation.

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  IP experiment of NIH/3T3 using 81857-1-RR (same clone as 81857-1-PBS)

  IP result of anti-PIN1 (IP:81857-1-RR, 4ug; Detection:81857-1-RR 1:20000) with NIH/3T3 cells lysate 1120 ug. This data was developed using the same antibody clone with 81857-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 81857-1-RR (same clone as 81857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81857-1-RR (PIN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81857-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 81857-1-RR (same clone as 81857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81857-1-RR (PIN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81857-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 81857-1-RR (same clone as 81857-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using PIN1 antibody (81857-1-RR, Clone: 5N20 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81857-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81857-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81857-1-RR against Human PIN1 were performed. The affinity constant is 0.17 nM.

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Informations sur le produit

81857-1-PBS cible PIN1 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PIN1 Protéine recombinante Ag0767
• Nom complet: peptidylprolyl cis/trans isomerase, NIMA-interacting 1
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC002899
• Symbole du gène: PIN1
• Identification du gène (NCBI): 5300
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PIN1(Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1) is essential for mitosis progression in yeast cells and is hypothesized to perform the same role in mammalian cells. It might regulate cellular processes distinct from the cell cycle itself, such as terminal differentiation through a modulation of differentiation-specific gene expression(PMID:20801874). It colocalizes with NEK6 in the nucleus. Pin1 inhibition simultaneously blocks multiple cancer pathways, disrupts the desmoplastic and immunosuppressive TME, and upregulates PD-L1 and ENT1, rendering pancreatic ductal adenocarcinoma (PDAC) eradicable by immunochemotherapy (PMID: 34388391).

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