Anticorps Polyclonal de lapin anti-PINK1

• Phare
• Validé par KD/KO

PINK1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

N° de cat : 23274-1-PBS

Synonymes

BRPK, EC:2.7.11.1, PARK6, PTEN induced putative kinase 1, PTEN-induced putative kinase protein 1

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Galerie de données de validation

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  WB analysis of HeLa using 23274-1-AP (same clone as 23274-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 23274-1-AP (PINK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

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  WB analysis using 23274-1-AP (same clone as 23274-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 23274-1-AP (PINK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

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  WB analysis using 23274-1-AP (same clone as 23274-1-PBS)

  Non-treated and CCCP treated HeLa cells were subjected to SDS PAGE followed by western blot with 23274-1-AP (PINK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 23274-1-AP (same clone as 23274-1-PBS)

  WB result of PINK1 antibody (23274-1-AP, 1:600) with si-Control and si-PINK1 transfected HeLa cells. This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 23274-1-AP (same clone as 23274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23274-1-AP (PINK1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 23274-1-AP (same clone as 23274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23274-1-AP (PINK1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 23274-1-AP (same clone as 23274-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 23274-1-AP (same clone as 23274-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 23274-1-AP (same clone as 23274-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 23274-1-AP (same clone as 23274-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23274-1-PBS in a different storage buffer formulation.

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Informations sur le produit

23274-1-PBS cible PINK1 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PINK1 Protéine recombinante Ag19825
• Nom complet: PTEN induced putative kinase 1
• Masse moléculaire calculée: 581 aa, 63 kDa
• Poids moléculaire observé: 65 kDa, 45 kDa
• Numéro d’acquisition GenBank: BC028215
• Symbole du gène: PINK1
• Identification du gène (NCBI): 65018
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PINK1 is a mitochondrial serine/threonine-protein kinase that protects cells from stress-induced mitochondrial dysfunction. The precursor of PINK1 (65 kDa) is synthesized in the cytosol and is imported into the outer membrane of mitochondria. PINK1 is further transferred into the inner membrane. The full-length PINK1 can be proteolytically processed into 52-55 kDa and 45-46 kDa forms (PMID: 18221368; 25108683; 18031932). The half-life of the mature form of PINK1 is very short and it was proposed that the proteasome is involved in its degradation (PMID: 23472196). The gene of PINK1 maps to chromosome 1p36.12. Two alternatively spliced variants exist, the shorter isoform (30 kDa) produced by alternative splicing. Mutations in the PINK1 gene cause autosomal recessive early-onset Parkinson's disease.

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