Anticorps Recombinant de lapin anti-PINK1

PINK1 Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240449E7

N° de cat : 81991-4-PBS

Synonymes

240449E7, BRPK, EC:2.7.11.1, PARK6, PTEN induced putative kinase 1

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Galerie de données de validation

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  WB analysis using 81991-4-RR (same clone as 81991-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81991-4-RR (PINK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81991-4-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 81991-4-RR (same clone as 81991-4-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug PINK1 Recombinant antibody (81991-4-RR, Clone:240449E7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81991-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81991-4-RR

  Biolayer interferometry (BLl) kinetic assays of 81991-4-RR against Human PINK1 were performed. The affinity constant is 125 nM.

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Informations sur le produit

81991-4-PBS cible PINK1 dans les applications de WB, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PINK1 Protéine recombinante Ag19825
• Nom complet: PTEN induced putative kinase 1
• Masse moléculaire calculée: 581 aa, 63 kDa
• Numéro d’acquisition GenBank: BC028215
• Symbole du gène: PINK1
• Identification du gène (NCBI): 65018
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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