Anticorps Polyclonal de lapin anti-PIP4K2A

• Phare
• Validé par KD/KO

PIP4K2A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 12469-1-PBS

Synonymes

Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha, Phosphatidylinositol 5-phosphate 4-kinase type II alpha, Phosphatidylinositol 5-Phosphate 4-Kinase, EC:2.7.1.149, Diphosphoinositide kinase 2-alpha

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Galerie de données de validation

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  WB analysis of mouse brain using 12469-1-AP (same clone as 12469-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 12469-1-AP (same clone as 12469-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

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  WB analysis using 12469-1-AP (same clone as 12469-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12469-1-AP (same clone as 12469-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 12469-1-AP (same clone as 12469-1-PBS)

  IP result of anti-PIP4K2A (IP:12469-1-AP, 4ug; Detection:12469-1-AP 1:500) with mouse brain tissue lysate 4400ug. This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 12469-1-AP (same clone as 12469-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12469-1-AP (PIP4K2A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 12469-1-AP (same clone as 12469-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12469-1-AP (PIP4K2A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 12469-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12469-1-AP (PIP4K2A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12469-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12469-1-AP (PIP4K2A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12469-1-AP (same clone as 12469-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PIP4K2A antibody (12469-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (black). This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 12469-1-AP (same clone as 12469-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 12469-1-AP (PIP4K2A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12469-1-PBS cible PIP4K2A dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PIP4K2A Protéine recombinante Ag3152
• Nom complet: phosphatidylinositol-5-phosphate 4-kinase, type II, alpha
• Masse moléculaire calculée: 406 aa, 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC018034
• Symbole du gène: PIP4K2A
• Identification du gène (NCBI): 5305
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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