Anticorps Recombinant de lapin anti-PIP4K2B

PIP4K2B Recombinant Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240645F10

N° de cat : 83647-3-PBS

Synonymes

Phosphatidylinositol 5-phosphate 4-kinase type-2 beta, Phosphatidylinositol 5-phosphate 4-kinase type II beta, EC:2.7.1.149, Diphosphoinositide kinase 2-beta, 240645F10

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Galerie de données de validation

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  WB analysis using 83647-3-RR (same clone as 83647-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83647-3-RR (PIP4K2B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83647-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83647-3-RR (same clone as 83647-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83647-3-RR (PIP4K2B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83647-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83647-3-RR (same clone as 83647-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83647-3-RR (PIP4K2B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83647-3-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 83647-3-RR (same clone as 83647-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using PIP4K2B antibody (83647-3-RR, Clone: 240645F10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83647-3-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 83647-3-RR (same clone as 83647-3-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug PIP4K2B Recombinant antibody (83647-3-RR, Clone:240645F10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83647-3-PBS in a different storage buffer formulation.

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Informations sur le produit

83647-3-PBS cible PIP4K2B dans les applications de WB, IHC, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PIP4K2B Protéine recombinante Ag4099
• Nom complet: phosphatidylinositol-5-phosphate 4-kinase, type II, beta
• Masse moléculaire calculée: 416 aa, 47 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC027459
• Symbole du gène: PIP4K2B
• Identification du gène (NCBI): 8396
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PIP4K2B, also named as PIP5K2B, participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. PIP4K2B has a homodimer.

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