Anticorps Polyclonal de lapin anti-PIP4K2C

• Phare
• Validé par KD/KO

PIP4K2C Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 17077-1-AP

Synonymes

PI(5)P 4 kinase type II gamma, PIP4K2C, PIP4KII gamma, PIP5K2C

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Galerie de données de validation

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  WB analysis using 17077-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17077-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17077-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17077-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17077-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17077-1-PBS

  HeLa cells were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17077-1-PBS

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 17077-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17077-1-AP (PIP4K2C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17077-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17077-1-AP (PIP4K2C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules PC-12
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below

Informations sur le produit

17077-1-AP cible PIP4K2C dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PIP4K2C Protéine recombinante Ag10989
• Nom complet: phosphatidylinositol-5-phosphate 4-kinase, type II, gamma
• Masse moléculaire calculée: 421 aa, 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC028596
• Symbole du gène: PIP4K2C
• Identification du gène (NCBI): 79837
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PIP4K2C is involved in several processes, including 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate biosynthetic process; negative regulation of 1-phosphatidylinositol-4-phosphate 5-kinase activity; and positive regulation of autophagosome assembly. PIP4Ks negatively regulate insulin signaling through a catalytic-independent mechanism.

Publications

Species	Application	Title
human	WB	Elife; Inhibition of PIP4Kγ ameliorates the pathological effects of mutant huntingtin protein.; Authors - Ismael Al-Ramahi ; KD Validated
human	WB	Cell Rep; PIP4Ks Suppress Insulin Signaling through a Catalytic-Independent Mechanism.; Authors - Diana G Wang ; KO Validated
human	WB	Autophagy Rep; Upregulation of the ESCRT pathway and multivesicular bodies accelerates degradation of proteins associated with neurodegeneration; Authors - Ron Benyair