Anticorps Polyclonal de lapin anti-PIP5K1A

• Phare
• Validé par KD/KO

PIP5K1A Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

12

Avis (1)

N° de cat : 15713-1-AP

Synonymes

PIP5K1 alpha, PIP5K1A, PIP5KIalpha, PtdIns(4)P 5 kinase 1 alpha

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Galerie de données de validation

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  WB analysis using 15713-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15713-1-AP (PIP5K1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15713-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15713-1-AP (PIP5K1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15713-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15713-1-AP (PIP5K1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 15713-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15713-1-AP (PIP5K1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa cells using 15713-1-AP

  IP result of anti-PIP5K1A (15713-1-AP for IP and Detection).

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  IP experiment of mouse heart using 15713-1-AP

  IP Result of anti-PIP5K1A (IP:15713-1-AP, 4ug; Detection:15713-1-AP 1:500) with mouse heart tissue lysate 3200ug.

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  IHC staining of human tonsillitis using 15713-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15713-1-AP (PIP5K1A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 15713-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15713-1-AP (PIP5K1A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15713-1-AP

  Immunofluorescent analysis of Hela cells, using PIP5K1A antibody 15713-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 15713-1-AP

  1X10^6 HeLa cells were stained with .5ug PIP5K1A antibody (15713-1-AP, red) and control antibody (blue). FITC-Goat anti-Rabbit IgG with dilution 1:100. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, cellules NCI-H1299, cellules PC-12, tissu cardiaque humain, tissu hépatique humain
• Résultats positifs en IP: cellules HeLa, tissu cardiaque de souris
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.05 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• IP: See 2 publications below

Informations sur le produit

15713-1-AP cible PIP5K1A dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PIP5K1A Protéine recombinante Ag8340
• Nom complet: phosphatidylinositol-4-phosphate 5-kinase, type I, alpha
• Masse moléculaire calculée: 56 kDa, 63 kDa
• Poids moléculaire observé: 63 kDa
• Numéro d’acquisition GenBank: BC007833
• Symbole du gène: PIP5K1A
• Identification du gène (NCBI): 8394
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) play diverse roles in the cellular biology of many organisms, including signal transduction, secretion and vesicular trafficking, and regulation of cytoskeleton assembly(PMID:17688436). There are three PIP5K isoforms,α, β, and γ. The nomenclature for the α and β isoforms is switched between humans and mice(PMID:22096541). There are also several splicing variants of the γ isozyme have been identified.(PMID:20945365). PIP5K1A has some isoforms produced by alternative splicing with the molecular mass of 56-63 kDa.

Publications

Species	Application	Title
rat	WB, IF	Nat Cell Biol; Clathrin and phosphatidylinositol-4,5-bisphosphate regulate autophagic lysosome reformation.; Authors - Rong Yueguang Y
human	WB	J Clin Invest; Spastic paraplegia proteins spastizin and spatacsin mediate autophagic lysosome reformation.; Authors - Jaerak Chang
human	WB	EMBO J; Phosphatidylinositol 4,5-bisphosphate controls Rab7 and PLEKHM1 membrane cycling during autophagosome-lysosome fusion.; Authors - Takashi Baba
human	WB,IF,IP,IHC	Oncogene; The role of PIP5K1α/pAKT and targeted inhibition of growth of subtypes of breast cancer using PIP5K1α inhibitor.; Authors - Martuza Sarwar ; KD Validated
mouse	WB	Nutrients; Effects of Gestational Diabetes Mellitus and Selenium Deficiency on the Offspring Growth and Blood Glucose Mechanisms of C57BL/6J Mice; Authors - Wenhui Xu
human	WB,IF	Front Cell Dev Biol; PIP5K1α is Required for Promoting Tumor Progression in Castration-Resistant Prostate Cancer.; Authors - Tianyan Wang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Bastien (Verified Customer) (08-19-2020)

Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,25 µl/ million of cells during 50min After, a seconde staining was performed with 0,1µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In red secondary antibody alone and in blue primary + secondary antibody

• Applications: Flow Cytometry
• Primary Antibody Dilution: 0,25µl/10^6cells
• Cell Tissue Type: B cells

PIP5K1A Antibody Flow Cytometry validation (0,25µl/10^6cells dilution) in B cells (Cat no:15713-1-AP)