Anticorps Monoclonal anti-PIP5K1A

PIP5K1A Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

3E5G12

N° de cat : 67483-1-Ig

Synonymes

PIP5K1 alpha, PIP5K1A, PIP5KIalpha, PtdIns(4)P 5 kinase 1 alpha

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Galerie de données de validation

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  WB analysis using 67483-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67483-1-Ig (PIP5K1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis using 67483-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67483-1-Ig (PIP5K1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67483-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67483-1-Ig (PIP5K1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 67483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67483-1-Ig (PIP5K1A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 67483-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67483-1-Ig (PIP5K1A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellule Hela, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules L02, cellules MOLT-4
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67483-1-Ig cible PIP5K1A dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PIP5K1A Protéine recombinante Ag8355
• Nom complet: phosphatidylinositol-4-phosphate 5-kinase, type I, alpha
• Masse moléculaire calculée: 56 kDa, 63 kDa
• Poids moléculaire observé: 63 kDa
• Numéro d’acquisition GenBank: BC007833
• Symbole du gène: PIP5K1A
• Identification du gène (NCBI): 8394
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) play diverse roles in the cellular biology of many organisms, including signal transduction, secretion and vesicular trafficking, and regulation of cytoskeleton assembly(PMID:17688436). There are three PIP5K isoforms,α, β, and γ. The nomenclature for the α and β isoforms is switched between humans and mice(PMID:22096541). There are also several splicing variants of the γ isozyme have been identified.(PMID:20945365). PIP5K1A has some isoforms produced by alternative splicing with the molecular mass of 56-63 kDa.

Publications

Species	Application	Title
human	WB	J Virol; The Rac1-PAK1-Arp2/3 signaling axis regulates CHIKV nsP1-induced filopodia and optimal viral genome replication; Authors - Olivier Aïqui-Reboul-Paviet

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