Anticorps Monoclonal anti-PITX2

• Phare
• Validé par KD/KO

PITX2 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

1E3B1

Avis (1)

N° de cat : 67201-1-Ig

Synonymes

1E3B1, ALL1-responsive protein ARP1, ARP1, Paired-like homeodomain transcription factor 2, RIEG bicoid-related homeobox transcription factor

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Galerie de données de validation

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  WB analysis using 67201-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67201-1-Ig (PITX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of HEK-293 using 67201-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67201-1-Ig (PITX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, cellules HEK-293, tissu cérébral de souris, tissu de cervelet de rat, tissu de cervelet de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

67201-1-Ig cible PITX2 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PITX2 Protéine recombinante Ag27924
• Nom complet: paired-like homeodomain 2
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC013998
• Symbole du gène: PITX2
• Identification du gène (NCBI): 5308
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PITX2 belongs to the bicoid class of homeodomain transcription factors, which play essential roles in embryonic development. Various PITX2 isoforms are involved in a wide variety of developmental programs, including left-right signaling. It controls cell proliferation in a tissue-specific manner and is involved in morphogenesis. During embryonic development, PITX2 is involved in the expansion of muscle progenitors. In addition, it may play a role in the proper localization of asymmetric organs such as the heart and stomach. PITX2 can be bound by some other proteins, and are part of a much larger atrial transcriptional complex. This antibody specifically recognizes isoform PTX2C.

Publications

Species	Application	Title
rat	WB	Oral Dis; Enamel defects of Axenfeld-Rieger syndrome and the role of PITX2 in its pathogenesis.; Authors - Yi Yang ; KD Validated
mouse	WB	Aging (Albany NY); D-galactose causes sinoatrial node dysfunction: from phenotype to mechanism; Authors - Heng Zhang ; KD Validated
mouse	IF	Genomics Proteomics Bioinformatics; Single-cell Atlas of Developing Mouse Palates Reveals Cellular and Molecular Transitions in Periderm Cell Fate; Authors - Wenbin Huang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kenzo (Verified Customer) (03-28-2024)

Worked well for immunofluorescence in mouse heart tissues.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse heart

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