Anticorps Polyclonal de lapin anti-PIWIL1

PIWIL1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 15659-1-AP

Synonymes

Piwi like protein 1, piwi like 1 (Drosophila), PIWI, MIWI, HIWI

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Galerie de données de validation

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  WB analysis of human testis using 15659-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15659-1-AP (PIWIL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15659-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15659-1-AP (PIWIL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 15659-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15659-1-AP (PIWIL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 15659-1-AP

  IP result of anti-PIWIL1 (IP:15659-1-AP, 4ug; Detection:15659-1-AP 1:2000) with mouse testis tissue lysate 2400 ug.

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  IHC staining of human testis using 15659-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 15659-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 15659-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 15659-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 15659-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 15659-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of mouse testis using 15659-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using PIWIL1 antibody (15659-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15659-1-AP

  Immunofluorescent analysis of HepG2 cells using 15659-1-AP (PIWIL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris,
• Résultats positifs en IHC: tissu testiculaire humain, tissu de cancer de la prostate humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu testiculaire de souris,
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Informations sur le produit

15659-1-AP cible PIWIL1 dans les applications de WB, IHC, IF/ICC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PIWIL1 Protéine recombinante Ag8056
• Nom complet: piwi-like 1 (Drosophila)
• Masse moléculaire calculée: 99 kDa
• Poids moléculaire observé: 85-95 kDa
• Numéro d’acquisition GenBank: BC028581
• Symbole du gène: PIWIL1
• Identification du gène (NCBI): 9271
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB,IF,IP	Development; MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis; Authors -
human	WB	Mol Ther Nucleic Acids; PIWIL1/piRNA-DQ593109 Regulates the Permeability of the Blood-Tumor Barrier via the MEG3/miR-330-5p/RUNX3 Axis.; Authors - Shuyuan Shen
mouse	WB,IF,IP	Development; MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis.; Authors - Xiaoli Wang
human	WB	J Transl Med; piRNA-14633 promotes cervical cancer cell malignancy in a METTL14-dependent m6A RNA methylation manner.; Authors - Qi Xie
human	IF	Prostate; High levels of PIWI-interacting RNAs are present in the small RNA landscape of prostate epithelium from vitamin D clinical trial specimens.; Authors - Bethany Baumann
mouse	WB	Andrologia; Secretions released from mesenchymal stem cells improve spermatogenesis restoration of cytotoxic treatment with busulfan in azoospermia mice.; Authors - Yi-Ting Cai

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