Anticorps Polyclonal de lapin anti-PJA1

• Phare
• Validé par KD/KO

PJA1 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 17687-1-AP

Synonymes

E3 ubiquitin-protein ligase Praja-1, EC:2.3.2.27, praja ring finger 1, Praja1, RING finger protein 70

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Galerie de données de validation

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  WB analysis using 17687-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17687-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17687-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 17687-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17687-1-AP

  IP result of anti-PJA1 (IP:17687-1-AP, 3ug; Detection:17687-1-AP 1:300) with mouse brain tissue lysate 3600ug.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

17687-1-AP cible PJA1 dans les applications de WB, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PJA1 Protéine recombinante Ag11820
• Nom complet: praja ring finger 1
• Masse moléculaire calculée: 643 aa, 71 kDa
• Poids moléculaire observé: 71-80 kDa
• Numéro d’acquisition GenBank: BC075803
• Symbole du gène: PJA1
• Identification du gène (NCBI): 64219
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PJA1(E3 ubiquitin-protein ligase Praja-1), also named as RNF70, regulates the level of PRC2 (polycomb repressive complex 2) by targeting its free subunits for Ub-mediated proteasomal degradation and it can be involved in protein ubiquitination in the brain and is a suitable candidate gene for MRX(PMID:12036302). The Praja1 protein is unlikely to be a membrane receptor, since it lacks a hydrophobic transmembrane domain(PMID:9393880). It has 3 isoforms produced by alternative splicing with the molecular weight of 71 kDa, 50 kDa and 65 kDa.

Publications

Species	Application	Title
mouse	WB,IF	Nat Commun; Praja1 E3 ubiquitin ligase promotes skeletal myogenesis through degradation of EZH2 upon p38α activation.; Authors - Silvia Consalvi ; KD Validated
human	WB	Ann Clin Transl Neurol; A recurrent PJA1 variant in trigonocephaly and neurodevelopmental disorders.; Authors - Toshimitsu Suzuki
mouse	WB	Biochim Biophys Acta Mol Cell Biol Lipids; Diacylglycerol kinase δ destabilizes serotonin transporter protein through the ubiquitin-proteasome system.; Authors - Qiang Lu
human	WB	J Virol; PJA1 Coordinates with the SMC5/6 Complex to Restrict DNA Viruses and Episomal Genes through Interferon-independent Manner.; Authors - Wei Xu
rat	WB	Neuropathology; Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal cytoplasmic TDP-43 aggregate formation.; Authors - Kazuhiko Watabe
rat	CoIP	Neuropathology; Praja1 RING-finger E3 ubiquitin ligase is a common suppressor of neurodegenerative disease-associated protein aggregation.; Authors - Kazuhiko Watabe

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