Anticorps Polyclonal de lapin anti-PJA1

• Phare
• Validé par KD/KO

PJA1 Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 17687-1-PBS

Synonymes

E3 ubiquitin-protein ligase Praja-1, EC:2.3.2.27, praja ring finger 1, Praja1, RING finger protein 70

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Galerie de données de validation

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  WB analysis using 17687-1-AP (same clone as 17687-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17687-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 17687-1-AP (same clone as 17687-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17687-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 17687-1-AP (same clone as 17687-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17687-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 17687-1-AP (same clone as 17687-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17687-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 17687-1-AP (same clone as 17687-1-PBS)

  IP result of anti-PJA1 (IP:17687-1-AP, 3ug; Detection:17687-1-AP 1:300) with mouse brain tissue lysate 3600ug. This data was developed using the same antibody clone with 17687-1-PBS in a different storage buffer formulation.

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Informations sur le produit

17687-1-PBS cible PJA1 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PJA1 Protéine recombinante Ag11820
• Nom complet: praja ring finger 1
• Masse moléculaire calculée: 643 aa, 71 kDa
• Poids moléculaire observé: 71-80 kDa
• Numéro d’acquisition GenBank: BC075803
• Symbole du gène: PJA1
• Identification du gène (NCBI): 64219
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PJA1(E3 ubiquitin-protein ligase Praja-1), also named as RNF70, regulates the level of PRC2 (polycomb repressive complex 2) by targeting its free subunits for Ub-mediated proteasomal degradation and it can be involved in protein ubiquitination in the brain and is a suitable candidate gene for MRX(PMID:12036302). The Praja1 protein is unlikely to be a membrane receptor, since it lacks a hydrophobic transmembrane domain(PMID:9393880). It has 3 isoforms produced by alternative splicing with the molecular weight of 71 kDa, 50 kDa and 65 kDa.

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