Anticorps Polyclonal de lapin anti-PKC Alpha

• Phare
• Validé par KD/KO

PKC Alpha Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21991-1-PBS

Synonymes

PRKCA, AAG6, EC:2.7.11.13, PKC A, PKCA

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Galerie de données de validation

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  WB analysis using 21991-1-AP (same clone as 21991-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21991-1-AP (PKC alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21991-1-PBS in a different storage buffer formulation.

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  WB analysis using 21991-1-AP (same clone as 21991-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21991-1-AP (PKC Alpha antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21991-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 21991-1-AP (same clone as 21991-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21991-1-AP (PKC alpha antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21991-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 21991-1-AP (same clone as 21991-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21991-1-AP (PKC alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21991-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 21991-1-AP (same clone as 21991-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21991-1-AP (PKC alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21991-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 21991-1-AP (same clone as 21991-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21991-1-AP (PKC alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21991-1-PBS in a different storage buffer formulation.

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  IP experiment of NIH/3T3 using 21991-1-AP (same clone as 21991-1-PBS)

  IP result of anti-PKC Alpha (IP:21991-1-AP, 4ug; Detection:21991-1-AP 1:3000) with NIH/3T3 cells lysate 1320 ug. This data was developed using the same antibody clone with 21991-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 21991-1-AP (same clone as 21991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21991-1-AP (PKC alpha antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 21991-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 21991-1-AP (same clone as 21991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21991-1-AP (PKC alpha antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 21991-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21991-1-AP (same clone as 21991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21991-1-AP (PKC Alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21991-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21991-1-AP (same clone as 21991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21991-1-AP (PKC Alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21991-1-PBS in a different storage buffer formulation.

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  IF Staining of rat eye using 21991-1-AP (same clone as 21991-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using PKC Alpha antibody (21991-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21991-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 21991-1-AP (same clone as 21991-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKC Alpha antibody (21991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21991-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 21991-1-AP (same clone as 21991-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PKC Alpha antibody (21991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21991-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 21991-1-AP (same clone as 21991-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 21991-1-AP (PKC alpha antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21991-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 21991-1-AP (same clone as 21991-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PKC Alpha (21991-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 21991-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 21991-1-AP (same clone as 21991-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug PKC Alpha Polyclonal antibody (21991-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 21991-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21991-1-PBS cible PKC Alpha dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PKC Alpha Protéine recombinante Ag17275
• Nom complet: protein kinase C, alpha
• Masse moléculaire calculée: 77 kDa
• Poids moléculaire observé: 77 kDa
• Numéro d’acquisition GenBank: AK055431
• Symbole du gène: PKC Alpha
• Identification du gène (NCBI): 5578
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PRKCA is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

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