Anticorps Polyclonal de lapin anti-PKC Delta

• Phare
• Validé par KD/KO

PKC Delta Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 19132-1-PBS

Synonymes

PRKCD, EC:2.7.10.2, EC:2.7.11.13, MAY1, nPKC-delta

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Galerie de données de validation

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  WB analysis using 19132-1-AP (same clone as 19132-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC Delta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  WB analysis using 19132-1-AP (same clone as 19132-1-PBS)

  Non-treated and PMA treated THP-1 cells were subjected to SDS PAGE followed by western blot with 29564-1-AP (Phospho-PKC delta (Thr507) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with PKC Delta antibody (19132-1-AP) subsequently. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse colon using 19132-1-AP (same clone as 19132-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC delta antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 19132-1-AP (same clone as 19132-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC delta antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 19132-1-AP (same clone as 19132-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC delta antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-1080 using 19132-1-AP (same clone as 19132-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC delta antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 19132-1-AP (same clone as 19132-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC delta antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  IP experiment of A431 using 19132-1-AP (same clone as 19132-1-PBS)

  IP result of anti-PKC Delta (IP:19132-1-AP, 4ug; Detection:19132-1-AP 1:800) with A431 cells lysate 2640 ug. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  IP experiment of A431 using 19132-1-AP (same clone as 19132-1-PBS)

  IP result of anti-PKC Delta (IP:19132-1-AP, 4ug; Detection:19132-1-AP 1:500) with A431 cells lysate 2000ug. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 19132-1-AP (same clone as 19132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19132-1-AP (PKC Delta antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 19132-1-AP (same clone as 19132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19132-1-AP (PKC Delta antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 19132-1-AP (same clone as 19132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 19132-1-AP (PKC delta antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 19132-1-AP (same clone as 19132-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 19132-1-AP (PKC delta antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 19132-1-AP (same clone as 19132-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 19132-1-AP (same clone as 19132-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 19132-1-AP (same clone as 19132-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 19132-1-AP (same clone as 19132-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 19132-1-AP (same clone as 19132-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PKC Delta (19132-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

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Informations sur le produit

19132-1-PBS cible PKC Delta dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PKC Delta Protéine recombinante Ag5631
• Nom complet: protein kinase C, delta
• Masse moléculaire calculée: 78 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC043350
• Symbole du gène: PKC Delta
• Identification du gène (NCBI): 5580
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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