Anticorps Recombinant de lapin anti-PKC Iota

PKC Iota Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

243126A4

N° de cat : 85733-2-PBS

Synonymes

PRKCI, aPKC lambda/iota, aPKC-lambda/iota, Atypical protein kinase C-lambda/iota, DXS1179E

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Galerie de données de validation

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  WB analysis using 85733-2-RR (same clone as 85733-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85733-2-RR (PRKCI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85733-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02074-3

  Sandwich ELISA standard curve of MP02074-3, Human PKC Iota Recombinant Matched Antibody Pair - PBS only. 85733-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4990. 85733-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

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  Sample test of MP02074-3

  The mean PKC Iota concentration was determined to be 47.20 ng/mL in HeLa cell extract based on a 3.0 mg/mL extract load.

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  Affinity and Kinetic Characterization of 85733-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85733-2-RR against Human PKC Iota were performed. The affinity constant is below 1 pM.

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Informations sur le produit

85733-2-PBS cible PKC Iota dans les applications de WB, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PKC Iota Protéine recombinante Ag4990
• Nom complet: protein kinase C, iota
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: BC022016
• Symbole du gène: PKC Iota
• Identification du gène (NCBI): 5584
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The atypical protein kinase C isoform PRKC iota (PRKCI) is a member of the protein kinase C (PKC) family of serine/threonine protein kinases. PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. PRKC iota is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. PRKC iota plays a key role in cell proliferation, differentiation, and carcinogenesis, and it has been shown to be a human oncogene.

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