Anticorps Monoclonal anti-PKC Iota

• Phare
• Validé par KD/KO

PKC Iota Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

3A1B6

N° de cat : 66493-1-Ig

Synonymes

PRKCI, 3A1B6, aPKC lambda/iota, aPKC-lambda/iota, Atypical protein kinase C-lambda/iota

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Galerie de données de validation

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  WB analysis using 66493-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC Iota antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HeLa using 66493-1-Ig

  WB result of PKC Iota antibody (66493-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKC Iota transfected HeLa cells.

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  WB analysis using 66493-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 66493-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66493-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of ROS1728 using 66493-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 66493-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 66493-1-Ig (PKC iota antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 66493-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66493-1-Ig (PKC iota antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66493-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66493-1-Ig (PKC iota antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human lung cancer using 66493-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using PKC Iota antibody (66493-1-Ig, Clone: 3A1B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 66493-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKC Iota antibody (66493-1-Ig, Clone: 3A1B6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules C6, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules LNCaP, cellules NIH/3T3, cellules RAW 264.7, cellules ROS1728
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du poumon humain,
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

66493-1-Ig cible PKC Iota dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PKC Iota Protéine recombinante Ag4990
• Nom complet: protein kinase C, iota
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC022016
• Symbole du gène: PKC Iota
• Identification du gène (NCBI): 5584
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The atypical protein kinase C isoform PRKC iota (PRKCI) is a member of the protein kinase C (PKC) family of serine/threonine protein kinases. PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. PRKC iota is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. PRKC iota plays a key role in cell proliferation, differentiation, and carcinogenesis, and it has been shown to be a human oncogene.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; Regulation of a PRMT5/NF-κB Axis by Phosphorylation of PRMT5 at Serine 15 in Colorectal Cancer.; Authors - Antja-Voy Hartley ; KD Validated

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