Anticorps Polyclonal de lapin anti-PKIA

PKIA Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11743-1-PBS

Synonymes

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Galerie de données de validation

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  IHC staining of mouse embryo using 11743-1-AP (same clone as 11743-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11743-1-AP (PKIA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11743-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse embryo using 11743-1-AP (same clone as 11743-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11743-1-AP (PKIA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11743-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 11743-1-AP (same clone as 11743-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11743-1-AP (PKIA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11743-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 11743-1-AP (same clone as 11743-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11743-1-AP (PKIA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11743-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11743-1-AP (same clone as 11743-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PKIA antibody (11743-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11743-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11743-1-PBS cible PKIA dans les applications de IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PKIA Protéine recombinante Ag2338
• Nom complet: protein kinase (cAMP-dependent, catalytic) inhibitor alpha
• Masse moléculaire calculée: 76 aa, 8 kDa
• Numéro d’acquisition GenBank: BC022265
• Symbole du gène: PKIA
• Identification du gène (NCBI): 5569
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

cAMP-dependent protein kinase inhibitor alpha (PKIA) is also named as PRKACN1, and belongs to the PKI family. Among them, PKIA, a member of protein kinase A (PKA) inhibitor family, was found to be most significantly and highly expressed in susceptible animals (PMID:24910983). Decreased PKIA signaling would directly impact PKA activation, potentially inducing DRP1 phosphorylation and increasing ER Ca2+ stores (PMID:32152556). miR-129-5p activated PKA to regulate the phosphorylation of beta-catenin and cAMP-response element binding protein (CREB) by inhibiting PKIA (PMID:36222334).

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