Anticorps Recombinant de lapin anti-PKLR

PKLR Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

251231F12

N° de cat : 86485-1-RR

Synonymes

EC:2.7.1.40, PK1, PKL, Pyruvate kinase isozymes L/R, Pyruvate kinase PKLR

---

Galerie de données de validation

• 

  WB analysis of HepG2 using 86485-1-RR

  HepG2 cells were subjected to SDS PAGE followed by western blot with 86485-1-RR (PKLR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 86485-1-RR

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 86485-1-RR (PKLR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 86485-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86485-1-RR against Human PKLR were performed. The affinity constant is below 1 pM.

---

Applications testées

• Résultats positifs en WB: cellules HepG2, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86485-1-RR cible PKLR dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PKLR Protéine recombinante Ag17933
• Nom complet: pyruvate kinase, liver and RBC
• Masse moléculaire calculée: 574 aa, 62 kDa
• Poids moléculaire observé: 58-62 kDa
• Numéro d’acquisition GenBank: BC025737
• Symbole du gène: PKLR
• Identification du gène (NCBI): 5313
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PKLR(Pyruvate kinase isozymes R/L) is also named as PK1,PKL,which is a glycolytic enzyme that catalyzes the transphosphorylation from phosphoenolpyruvate (PEP) to ADP, yielding pyruvate and ATP. It is the last step of the glycolytic pathway and is essentially irreversible.It belongs to the pyruvate kinase family and There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues.Defects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) and pyruvate kinase deficiency of red cells (PKRD).It can form a homotetramer(PMID:11960989).

{{ptg:RelatedPrimaryAntibodies}}