Anticorps Polyclonal de lapin anti-PKM1-specific

• Phare
• Validé par KD/KO

PKM1-specific Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15821-1-PBS

Synonymes

PKM, Pyruvate kinase PKM, OIP 3, EC:2.7.11.1, EC:2.7.10.2

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Galerie de données de validation

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  WB analysis using 15821-1-AP (same clone as 15821-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 15821-1-AP (same clone as 15821-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 15821-1-AP (same clone as 15821-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

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  WB analysis using 15821-1-AP (same clone as 15821-1-PBS)

  rat brain tissue lysates were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 15821-1-AP (same clone as 15821-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 15821-1-AP (same clone as 15821-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

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  WB analysis of multi-cells/tissue using 15821-1-AP (same clone as 15821-1-PBS)

  WB results of 15821-1-AP (PKM1 antibody) at dilution of 1:1000. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse muscle/liver tissues using 15821-1-AP (same clone as 15821-1-PBS)

  WB results of PKM1,PKM2,PKLR on mouse muscle tissue and mouse liver tissue. (anti-PKM2 antibody is used as a negative control for mouse muscle and liver. Anti-PKM1 antibody is used as a negative control for mouse liver. ). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart tissue using 15821-1-AP (same clone as 15821-1-PBS)

  WB results of PKM1,PKM2,PKLR on mouse heart tissue. (anti-PKM1 antibody is used as a negative control.). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

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  WB analysis of 293 cell using 15821-1-AP (same clone as 15821-1-PBS)

  WB results of PKM1,PKM2,PKLR on 293 cell. (anti-PKM1 antibody is used as a negative control). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2/MCF7 cells using 15821-1-AP (same clone as 15821-1-PBS)

  WB results of PKM1,PKM2,PKLR on hepg2 cell and MCF7 cell. (anti-PKM1 antibody is used as a negative control.). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 15821-1-AP (same clone as 15821-1-PBS)

  IP result of anti-PKM1-specific (IP:15821-1-AP, 4ug; Detection:15821-1-AP 1:40000) with mouse brain tissue lysate 1680 ug. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 15821-1-AP (same clone as 15821-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 15821-1-AP (same clone as 15821-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 15821-1-AP (same clone as 15821-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 15821-1-AP (same clone as 15821-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 15821-1-AP (same clone as 15821-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 15821-1-AP (same clone as 15821-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 15821-1-AP (same clone as 15821-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 15821-1-AP (same clone as 15821-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15821-1-AP (PKM1-specific antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 15821-1-AP (same clone as 15821-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15821-1-AP (PKM1-specific antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 15821-1-AP (same clone as 15821-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 15821-1-AP (same clone as 15821-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15821-1-PBS cible PKM1-specific dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: PKM pyruvate kinase, muscle
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: NM_182470
• Symbole du gène: PKM
• Identification du gène (NCBI): 5315
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named PKM1 and PKM2. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). This antibody is specific to PKM1 isoform.

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