Anticorps Monoclonal anti-PKM2-specific

• Phare
• Validé par KD/KO

PKM2-specific Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

1C11C7

N° de cat : 60268-1-PBS

Synonymes

PKM2, PK-M2, PKM2 pyruvate kinase, muscle, PKM, Pyruvate kinase PKM

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Galerie de données de validation

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  WB analysis of HEK-293 using 60268-1-Ig (same clone as 60268-1-PBS)

  WB result of PKM2 antibody (60268-1-Ig, 1:3000) with si-Control and si-PKM2 transfected HEK293 cells. This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  WB analysis using 60268-1-Ig (same clone as 60268-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 60268-1-Ig (same clone as 60268-1-PBS)

  Western blot analysis of PK-M2-specific in various lines using Proteintech antibody 60268-1-Ig at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 60268-1-Ig (same clone as 60268-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 60268-1-Ig (same clone as 60268-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 60268-1-Ig (same clone as 60268-1-PBS)

  IP result of anti-PKM2-specific (IP:60268-1-Ig, 5ug; Detection:15822-1-AP (PKM2-specific rabbit poly-antibody), 1:300) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60268-1-Ig (same clone as 60268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60268-1-Ig (PKM2-specific antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60268-1-Ig (same clone as 60268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60268-1-Ig (PKM2-specific antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60268-1-Ig (same clone as 60268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60268-1-Ig (same clone as 60268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60268-1-Ig (same clone as 60268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:0 (under 10x lens). This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60268-1-Ig (same clone as 60268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:0 (under 40x lens). This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60268-1-Ig (same clone as 60268-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PKM2-specific antibody (60268-1-Ig, Clone: 1C11C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60268-1-Ig (same clone as 60268-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 60268-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60268-1-PBS cible PKM2-specific dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: PKM pyruvate kinase, muscle
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: NM_002654
• Symbole du gène: PKM
• Identification du gène (NCBI): 5315
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms,the activity of the M2 isoform can be inhibited by tyrosine kinase signalling in tumourcells. The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform.

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