Anticorps Polyclonal de lapin anti-PKMYT1

• Phare
• Validé par KD/KO

PKMYT1 Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29489-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of MCF-7 using 29489-1-AP (same clone as 29489-1-PBS)

  WB result of PKMYT1 antibody (29489-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKMYT1 transfected MCF-7 cells. This data was developed using the same antibody clone with 29489-1-PBS in a different storage buffer formulation.

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  WB analysis using 29489-1-AP (same clone as 29489-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29489-1-AP (PKMYT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29489-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29489-1-PBS cible PKMYT1 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PKMYT1 Protéine recombinante Ag30573
• Nom complet: protein kinase, membrane associated tyrosine/threonine 1
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 60-75 kDa
• Numéro d’acquisition GenBank: NM_004203
• Symbole du gène: PKMYT1
• Identification du gène (NCBI): 9088
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PKMYT1, also known as MYT1, belongs to the WEE1 family. PKMYT1 functions to negatively regulate the Cdc2-cyclin B complexes by phosphorylating Cdc2 on threonine 14 and tyrosine 15. PKMYT1 has also been implicated in an increasing number of cancer types, including gastric cancer, non-small-cell lung cancer, hepatocellular carcinoma, glioblastoma, neuroblastoma, and colorectal cancer, in which overexpression of PKMYT1 generally correlates with poor prognosis and disease progression. PKMYT1 also has another key role in the cell cycle - regulating Golgi membrane reassembly following mitosis - and depletion of PKMYT1 leads to cell death. PKMYT1 was strongly phosphorylated during mitosis. This antibody can recognize interphase and mitotic phase PKMYT1. (PMID: 9268380, 32127662)

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