Anticorps Polyclonal de lapin anti-PKN2

• Phare
• Validé par KD/KO

PKN2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 14608-1-AP

Synonymes

PRKCL2, PRK2, PKN gamma, PAK2, Pak 2

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Galerie de données de validation

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  WB analysis of HEK-293 using 14608-1-AP

  WB result of PKN2 antibody (14608-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKN2 transfected HEK-293 cells.

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  WB analysis using 14608-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14608-1-AP (PKN2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 14608-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 14608-1-AP (PKN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14608-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14608-1-AP (PKN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14608-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14608-1-AP (PKN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14608-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14608-1-AP (PKN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 14608-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14608-1-AP (PKN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 14608-1-AP

  IP result of anti-PKN2 (IP:14608-1-AP, 4ug; Detection:14608-1-AP 1:1000) with COLO 320 cells lysate 2560 ug.

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  IHC staining of human colon cancer using 14608-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14608-1-AP (PKN2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human stomach using 14608-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14608-1-AP (PKN2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14608-1-AP

  Immunofluorescent analysis of HepG2 cells, using PKN2 antibody 14608-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 14608-1-AP

  Immunofluorescent analysis of Hela cells, using PKN2 antibody 14608-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HepG2 using 14608-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PKN2 (14608-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A2780, cellules COLO 320, cellules HEK-293, cellules HEK-293T, cellules HeLa, cellules K-562, cellules SKOV-3
• Résultats positifs en IP: cellules COLO 320,
• Résultats positifs en IHC: tissu d'estomac humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

14608-1-AP cible PKN2 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PKN2 Protéine recombinante Ag6164
• Nom complet: protein kinase N2
• Masse moléculaire calculée: 112 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC062620
• Symbole du gène: PKN2
• Identification du gène (NCBI): 5586
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cancer Cell Int; Upregulation of SNTB1 correlates with poor prognosis and promotes cell growth by negative regulating PKN2 in colorectal cancer; Authors - Liya Liu
pig	WB,IHC	Reprod Domest Anim; miR-26a inhibits proliferation and promotes apoptosis in porcine immature Sertoli cells by targeting the PAK2 gene.; Authors - Maoliang Ran
human	ChIP	Mol Med; PKN2 enhances the immunosuppressive activity of polymorphonuclear myeloid-derived suppressor cells in esophageal carcinoma by mediating fatty acid oxidation; Authors - Xiao Fu

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