Anticorps Polyclonal de lapin anti-Plakophilin 1

Plakophilin 1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 22632-1-PBS

Synonymes

PKP1, Plakophilin-1, B6P

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Galerie de données de validation

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  WB analysis using 22632-1-AP (same clone as 22632-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22632-1-AP (PKP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22632-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 22632-1-AP (same clone as 22632-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 22632-1-AP (PKP1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22632-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 22632-1-AP (same clone as 22632-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using PKP1 antibody (22632-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 22632-1-PBS in a different storage buffer formulation.

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Informations sur le produit

22632-1-PBS cible Plakophilin 1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Plakophilin 1 Protéine recombinante Ag18153
• Nom complet: plakophilin 1 (ectodermal dysplasia/skin fragility syndrome)
• Masse moléculaire calculée: 747 aa, 83 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC114571
• Symbole du gène: PKP1
• Identification du gène (NCBI): 5317
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Plakophilin 1, also known as PKP1, B6P, is a member of the arm-repeat protein family. PKP1 contains arm-repeat (armadillo) domains, localizes to nuclei and cell desmosomes, and participates in linking cadherins to intermediate filaments in the cytoskeleton (PMID: 10747098). PKP1 contributes to epidermal morphogenesis and facilitates the formation of intermediate filaments. Mutations in PKP1 have been associated with the ectodermal dysplasia/skin fragility syndrome (PubMed:9326952, 10852826).

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