Anticorps Polyclonal de lapin anti-PLA2G12A

PLA2G12A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16009-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse skeletal muscle using 16009-1-AP (same clone as 16009-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16009-1-AP (PLA2G12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16009-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 16009-1-AP (same clone as 16009-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16009-1-AP (PLA2G12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16009-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 16009-1-AP (same clone as 16009-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16009-1-AP (PLA2G12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16009-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 16009-1-AP (same clone as 16009-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16009-1-AP (PLA2G12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16009-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 16009-1-AP (same clone as 16009-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16009-1-AP (PLA2G12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16009-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 16009-1-AP (same clone as 16009-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16009-1-AP (PLA2G12A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16009-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 16009-1-AP (same clone as 16009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16009-1-AP (PLA2G12A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16009-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 16009-1-AP (same clone as 16009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16009-1-AP (PLA2G12A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16009-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 16009-1-AP (same clone as 16009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 16009-1-AP (PLA2G12A Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 16009-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 16009-1-AP (same clone as 16009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 16009-1-AP (PLA2G12A Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 16009-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 16009-1-AP (same clone as 16009-1-PBS)

  Immunofluorescent analysis of HEK-293 cells using 16009-1-AP (PLA2G12A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16009-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16009-1-PBS cible PLA2G12A dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLA2G12A Protéine recombinante Ag8722
• Nom complet: phospholipase A2, group XIIA
• Masse moléculaire calculée: 189 aa, 21 kDa
• Poids moléculaire observé: 21-24 kDa
• Numéro d’acquisition GenBank: BC017218
• Symbole du gène: PLA2G12A
• Identification du gène (NCBI): 81579
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PLA2G12A (or PLA2G12) gene encodes group XII secreted phospholipase A2 (sPLA2) which catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Cellular arachidonate (AA) release and prostaglandin (PG) production is regulated by sPLA(2)s, groups III and XII. Group XII sPLA2 have relatively low specific activity and are structurally and functionally distinct from other sPLA2s. Cells transfected with group XII sPLA(2) exhibited abnormal morphology, suggesting a unique functional aspect of this enzyme. Role of sPLA2s as potential tumour biomarkers and therapeutic targets for various cancers have been reported.

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