Anticorps Polyclonal de lapin anti-PLA2G1B

PLA2G1B Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15843-1-PBS

Synonymes

PLA2, Phosphatidylcholine 2-acylhydrolase 1B, EC:3.1.1.4

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Galerie de données de validation

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  WB analysis using 15843-1-AP (same clone as 15843-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15843-1-AP (PLA2G1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15843-1-PBS in a different storage buffer formulation.

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  WB analysis using 15843-1-AP (same clone as 15843-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15843-1-AP (PLA2G1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15843-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse spleen using 15843-1-AP (same clone as 15843-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15843-1-AP (PLA2G1B Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 15843-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 15843-1-AP (same clone as 15843-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15843-1-AP (PLA2G1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15843-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 15843-1-AP (same clone as 15843-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15843-1-AP (PLA2G1B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15843-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse pancreas using 15843-1-AP (same clone as 15843-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using PLA2G1B antibody (15843-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15843-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15843-1-PBS cible PLA2G1B dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLA2G1B Protéine recombinante Ag8603
• Nom complet: phospholipase A2, group IB (pancreas)
• Masse moléculaire calculée: 70aa,8 kDa; 148aa,16 kDa
• Poids moléculaire observé: 13-16 kDa
• Numéro d’acquisition GenBank: BC005386
• Symbole du gène: PLA2G1B
• Identification du gène (NCBI): 5319
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PLA2G1B is a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. PLA2G1B catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids.

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