Anticorps Polyclonal de lapin anti-PLA2G4E

• Phare
• Validé par KD/KO

PLA2G4E Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 18088-1-AP

Synonymes

phospholipase A2, group IVE, PLA2G4E Ens

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Galerie de données de validation

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  WB analysis of SH-SY5Y using 18088-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18088-1-AP (PLA2G4E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 18088-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18088-1-AP (PLA2G4E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 18088-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18088-1-AP (PLA2G4E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SW 1990 using 18088-1-AP

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 18088-1-AP (PLA2G4E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 18088-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 18088-1-AP (PLA2G4E antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 18088-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 18088-1-AP (PLA2G4E antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 18088-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18088-1-AP (PLA2G4E antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 18088-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18088-1-AP (PLA2G4E antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 18088-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PLA2G4E antibody (18088-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules NIH/3T3, cellules SH-SY5Y, cellules SW-1990, tissu de thymus de souris
• Résultats positifs en IHC: tissu de muscle squelettique humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:800-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

18088-1-AP cible PLA2G4E dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLA2G4E Protéine recombinante Ag12808
• Nom complet: phospholipase A2, group IVE
• Masse moléculaire calculée: 868 aa, 99 kDa
• Poids moléculaire observé: 99 kDa, 57 kDa
• Numéro d’acquisition GenBank: BC101584
• Symbole du gène: PLA2G4E
• Identification du gène (NCBI): 123745
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PLA2G4E (Phospholipase A2 group IVE), also known as cPLA2-epsilon. Calcium-dependent N-acyltransferase is involved in the biosynthesis of N-acyl ethanolamines (NAEs) in the brain (PMID:29447909). Transfers the sn-1 fatty acyl chain of phosphatidylcholine (fatty acyl donor) to the amine group of phosphatidylethanolamine (fatty acyl acceptor) to generate N-acyl phosphatidylethanolamine (NAPE). Similarly can use plasmenylethanolamine as a fatty acyl acceptor to form N-acyl plasmenylethanolamine (N-Acyl-PlsEt). Both NAPE and N-Acyl-PlsEt can serve as precursors of bioactive NAEs like N-arachidonoyl phosphatidylethanolamine also called anandamide (PMID:29447909; 30517655).

Publications

Species	Application	Title
mouse	WB	Autophagy; Inhibition of PLA2G4E/cPLA2 promotes survival of random skin flaps by alleviating Lysosomal membrane permeabilization-Induced necroptosis.; Authors - Junsheng Lou ; KD Validated
mouse,human	WB,IHC,IF	Prog Neurobiol; PLA2G4E, a candidate gene for resilience in Alzheimer´s disease and a new target for dementia treatment.; Authors - Marta Pérez-González ; KD Validated
mouse	WB	FASEB J; Group IVE cytosolic phospholipase A2 limits psoriatic inflammation by mobilizing the anti-inflammatory lipid N-acylethanolamine.; Authors - Luyiyun Liang
mouse	WB	Front Genet; Deletion of the Capn1 Gene Results in Alterations in Signaling Pathways Related to Alzheimer's Disease, Protein Quality Control and Synaptic Plasticity in Mouse Brain.; Authors - Wenyue Su
mouse	WB	J Neuroinflammation; Elamipretide alleviates pyroptosis in traumatically injured spinal cord by inhibiting cPLA2-induced lysosomal membrane permeabilization; Authors - Haojie Zhang
mouse	WB	Skin Res Technol; Establishment of a Mouse Model for Porokeratosis Due to Mevalonate Diphosphate Decarboxylase Deficiency; Authors - Kexin Peng

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