Anticorps Polyclonal de lapin anti-PLA2G4E

• Phare
• Validé par KD/KO

PLA2G4E Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18088-1-PBS

Synonymes

phospholipase A2, group IVE, PLA2G4E Ens

---

Galerie de données de validation

• 

  WB analysis of SH-SY5Y using 18088-1-AP (same clone as 18088-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18088-1-AP (PLA2G4E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18088-1-AP (same clone as 18088-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18088-1-AP (PLA2G4E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SW 1990 using 18088-1-AP (same clone as 18088-1-PBS)

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 18088-1-AP (PLA2G4E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 18088-1-AP (same clone as 18088-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18088-1-AP (PLA2G4E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 18088-1-AP (same clone as 18088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 18088-1-AP (PLA2G4E antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 18088-1-AP (same clone as 18088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 18088-1-AP (PLA2G4E antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 18088-1-AP (same clone as 18088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 18088-1-AP (PLA2G4E antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 18088-1-AP (same clone as 18088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 18088-1-AP (PLA2G4E antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 18088-1-AP (same clone as 18088-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PLA2G4E antibody (18088-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

18088-1-PBS cible PLA2G4E dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLA2G4E Protéine recombinante Ag12808
• Nom complet: phospholipase A2, group IVE
• Masse moléculaire calculée: 868 aa, 99 kDa
• Poids moléculaire observé: 99 kDa, 57 kDa
• Numéro d’acquisition GenBank: BC101584
• Symbole du gène: PLA2G4E
• Identification du gène (NCBI): 123745
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PLA2G4E (Phospholipase A2 group IVE), also known as cPLA2-epsilon. Calcium-dependent N-acyltransferase is involved in the biosynthesis of N-acyl ethanolamines (NAEs) in the brain (PMID:29447909). Transfers the sn-1 fatty acyl chain of phosphatidylcholine (fatty acyl donor) to the amine group of phosphatidylethanolamine (fatty acyl acceptor) to generate N-acyl phosphatidylethanolamine (NAPE). Similarly can use plasmenylethanolamine as a fatty acyl acceptor to form N-acyl plasmenylethanolamine (N-Acyl-PlsEt). Both NAPE and N-Acyl-PlsEt can serve as precursors of bioactive NAEs like N-arachidonoyl phosphatidylethanolamine also called anandamide (PMID:29447909; 30517655).

{{ptg:RelatedPrimaryAntibodies}}