Anticorps Polyclonal de lapin anti-PLAA

• Phare
• Validé par KD/KO

PLAA Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12529-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis of MCF-7 using 12529-1-AP (same clone as 12529-1-PBS)

  WB result of PLAA antibody (12529-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLAA transfected MCF-7 cells. This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 12529-1-AP (same clone as 12529-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 12529-1-AP (PLAA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 12529-1-AP (same clone as 12529-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12529-1-AP (PLAA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

• 

  IP experiment of K-562 using 12529-1-AP (same clone as 12529-1-PBS)

  IP result of anti-PLAA (IP:12529-1-AP, 4ug; Detection:12529-1-AP 1:600) with K-562 cells lysate 3200ug. This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 12529-1-AP (same clone as 12529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 12529-1-AP (same clone as 12529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 12529-1-AP (same clone as 12529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 12529-1-AP (same clone as 12529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

12529-1-PBS cible PLAA dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PLAA Protéine recombinante Ag3224
• Nom complet: phospholipase A2-activating protein
• Masse moléculaire calculée: 738 aa, 81 kDa
• Poids moléculaire observé: 72-74 kDa
• Numéro d’acquisition GenBank: BC032551
• Symbole du gène: PLAA
• Identification du gène (NCBI): 9373
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Phospholipase A-2-activating protein (PLAA), also named PLAP, can interact with ubiquitin and is involved in the maintenance of ubiquitin levels. PLAA contains multiple consensus domains including WD repeats, PFU domain and PUL domain. Although the function of PLAA remains unclear, ubiquitin binding of PLAA might be the central role that connects ubiquitination and degradation in ERAD. Recent finding revealed that PLAA served as a novel nociveptive mediator after incision, and its expression level is regulated by miR-203.

{{ptg:RelatedPrimaryAntibodies}}