Anticorps Monoclonal anti-PLAA

• Phare
• Validé par KD/KO

PLAA Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5C10G9

N° de cat : 60244-1-PBS

Synonymes

5C10G9, DOA1, PLA2P, PLAP

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Galerie de données de validation

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  WB analysis of MCF-7 using 60244-1-Ig (same clone as 60244-1-PBS)

  WB result of PLAA antibody (60244-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLAA transfected MCF-7 cells. This data was developed using the same antibody clone with 60244-1-PBS in a different storage buffer formulation.

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  WB analysis of ROS1728 using 60244-1-Ig (same clone as 60244-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60244-1-Ig (PLAA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60244-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 60244-1-Ig (same clone as 60244-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 60244-1-Ig (PLAA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60244-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 60244-1-Ig (same clone as 60244-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 60244-1-Ig (PLAA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60244-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 60244-1-Ig (same clone as 60244-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60244-1-Ig (PLAA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60244-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60244-1-Ig (same clone as 60244-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60244-1-Ig (PLAA Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60244-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60244-1-Ig (same clone as 60244-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60244-1-Ig (PLAA Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60244-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60244-1-PBS cible PLAA dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PLAA Protéine recombinante Ag3224
• Nom complet: phospholipase A2-activating protein
• Masse moléculaire calculée: 738 aa, 81 kDa
• Poids moléculaire observé: 75-80 kDa
• Numéro d’acquisition GenBank: BC032551
• Symbole du gène: PLAA
• Identification du gène (NCBI): 9373
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Phospholipase A-2-activating protein (PLAA), also named PLAP, can interact with ubiquitin and is involved in the maintenance of ubiquitin levels. PLAA contains multiple consensus domains including WD repeats, PFU domain and PUL domain. Although the function of PLAA remains unclear, ubiquitin binding of PLAA might be the central role that connects ubiquitination and degradation in ERAD. Recent finding revealed that PLAA served as a novel nociveptive mediator after incision, and its expression level is regulated by miR-203.

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